Hi Melanie, You could look at Groeneveld K., te Marvelde JG, van den Beemd M.W., Hooijkaas H. & van Dongen J.J. Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes. Leukemia 1996; 10: 1383-1389 and Kappelmayer J, Gratama JW, Karaszi E, Menendez P, Ciudad J, Rivas R, Orfao A. Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols. J Immunol Methods. 2000 Aug 28;242(1-2):53-65. for ideas. Kind regards Anna At 16:58 2000-09-25 -0700, you wrote: > >I am doing parallel studies of Fix & Perm, Intraprep, and Facs Lyse for >intracellular staining of MPO, Tdt, and light chains (plasma cells). I am >looking for tips on reagents, procedures, antibody combinations, preferred >manufacturer of antibodies, gating techniques and any other useful >information. Thanks fellow flowers! Melanie O'Donahue > Anna Porwit Hematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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