John R. Kuhnmuench III asked: "I am in search of a cell line that may be a model for human neutrophil apoptosis. Does one exist? If so where could I find it? Thanks, John" John R. Kuhnmuench III Medical College of Wisconsin ======================================== You can use HL60 cells - there are a number of procedures you can use. We have a short review on some of these procedures which you can find from Methods in Cell Biology book below You can download a copy of the chapter from our website http://www.cyto.purdue.edu/flowcyt/research/pub1.htm or buy the book!! Functional Measurements Using HL-60 Cells. In Reference: Z. Darzynkiewicz, J. P. Robinson, and H. Crissman (Eds.), Methods in Cell Biology, Vol 41, p.p. 423-436. Academic Press, 1994. Best regards Paul Robinson <nofill> J.Paul Robinson, Ph.D., Professor of Immunopharmacology Professor of Biomedical Engineering Director, Purdue University Cytometry Laboratories Hansen Hall, Roon B050 Purdue University, West Lafayette, IN 47907-1515 (Ph) (765) 494-0757 Fax (765) 494-0517 jpr@flowcyt.cyto.purdue.edu http://www.cyto.purdue.edu http://www.bioscope.org
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:33 EST