Hello, we have this kind of request from time to time, mainly in patients with monoclonal gammapathy and approx. 8-10% of plasma cells in BM smears. We use two basic triple stainings: CD38/F/CD56PE/CD19TRI and cylambdaF/cykappaF/CD138, where we use Intra-Stain from Dako for cyt.Ig. We acquire 50.000 events and during analysis gate on CD38bright in the first tube and on CD138 in the second. Then we look for the normal vs pathological plasma cells and for kappa/lambda ratio. kind regards Anna At 11:27 2000-09-19 -0400, you wrote: > >I recently came across the article published in the AM J Pathol (1998, >152:1655-1665) by Ocqueteau et al regarding the immunophenotype of plasma >cells from monoclonal gammopathy of undetermined significance. The >following questions are for those of you that work in a clinical setting. > >Do you receive requests for typing (by flow cytometry) plasma cells in bone >marrow of patients with MGUS? >Do you offer the service? >If so what protocol do you fallow? > >I will greatly appreciate your assistance. > >Leonel W. Edwards, MD. >Assistant Medical Director >Department of Pathology and Laboratory Medicine >Danbury Hospital > > Anna Porwit Hematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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