> Research Triangle Cytometry Association > Meeting Announcement > > > "High resolution immunophenotyping of lymphocytic cells by enzymatic > amplification staining (EAS)" > > David Kaplan, M.D./Ph.D. > Professor of Pathology > Case Western Reserve University > Cleveland, Ohio > > Abstract: Flow cytometric analysis is a powerful technique for the single > cell > assessment of cell surface expression of selected molecules. The major > deficiency of flow cytometry has been its relative insensitivity. Only > molecules expressed in abundance have been readily observed. > Consequently, > we have developed an enzymatic amplification procedure for the analysis of > cell surface molecules by flow cytometry. Transformed and nontransformed > cells expressing MHC class I, CD5, CD3, CD4, CD6, CD7, CD34, CD45, MHC > class II, Fas ligand, and phosphatidylserine were assessed. Our enzymatic > amplification technology increased the fluorescence signal between 10 and > 100 fold for all surface molecules tested. Enzymatic amplification > staining (EAS) produces a significant enhancement in the resolving power > of > flow cytometric analysis of cell surface molecules. Using this technique > we have been able to detect the presence of molecules that could not be > observed by the standard procedure. > > Tuesday, September 26, 2000 > 9:00 AM > > Duke North Hospital > Lecture Hall 2001 > Durham, NC > > Product information from Flow-Amp Systems will be available after the > presentation. > > Park in the deck on Erwin Road across from Duke North Hospital. Walk > across the street through the main entrance to the hospital and take the > elevators to the 2nd floor. Proceed straight toward the front entrance on > the elevated walkway to Room 2001. > > We gratefully acknowledge Duke University Comprehensive Cancer Center for > reimbursing the expenses of this meeting. For more information contact > Mike Cook (919) 613-7819 or cook0016@mc.duke.edu. > > Cheers, > Sam > Sam Witherspoon > sw11527@glaxowellcome.com > > Dept. of Receptor Biochemistry Tel. 919-483-3078 > Glaxo Wellcome R&D Page 919-857-7768 > 5 Moore Dr. Fax 919-483-0585 > RTP, NC 27709 >
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