Dear Flowers, Is it safe to use Rho 123 to estimate the mitochondrial transmembrane potential? If yes, what is the ideal Rho 123 concentration? I have found a large difference on the literature (from 5uM to 50nM). Can I use CCCP (10uM) and Nigericin (10uM) as control? Thank you all in advance. Luis Fernando Marques-Santos ¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬ Luis Fernando Marques-Santos Laboratório de Imunologia Tumoral Dept. de Bioquímica Médica CCS - ICB - UFRJ - 21941-590 Rio de Janeiro - Brazil e-mail: marques-santos@olimpo.com.br fax: 55 21 270 8647 tel: 55 21 560 88 95 ext. 151 ¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬
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