> >Why not try staining for intranuclear cyclin D1 to determine if it is mantle cell >lymphoma? ---------- Hi and thanks to all who wrote to give opinions and suggestions. The one above, to run Cyclin D1, was the most commonly cited way to solve the given problem of differentiating between CLL and Mantle Cell. We did do cyclin D1 on the tissue originally and it was negative. Our protocol (IHC) for this particular stain, as I've explained to others, is not one that I'd stand by because I can't get the staining quite right on known mantle cells (though it's just fine on breast tissues). Cyclin D1 was also performed on the bone marrow by the _other_ lab - it, too, was negative. Cytogenetics (bone marrow) showed a trisomy 12 in one cell. The clinicians are going to treat the case as a CLL, but more aggressively than they would a "typical" CLL. The question below is interesting, though. Is CLL _ever_ cyclin D1 positive? >>Or if it is cyclin D1 positive, would anyone consider the term "atypical CLL"? Does >>this have any meaning? Thanks again for coming to my aid!! sharon
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:31 EST