Dear Flowers, Once again I was greatly helped by all your responses to my COS7 cell cycle analysis question. As it turns out COS7 is apparently not double nucleated. The consensus of your responses was that I had two cycling populations. I reanalyzed the data and feel much more confident of the analysis. I have not heard back from the investigator for feedback about his cells, so don't have that final piece. Again, thank you for being such a valuable resource. Candace Enockson Medical University of South Carolina
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