The BDIS anti-cytokine antibodies work with the BD Lysing Solution and the BD Perm Solution. Even if you use PBMC and not whole blood you have to use the Lysing solution to fix the cells before staining. In contrast, some of the BD Pharmingen antibodies require their Pharmingen Fix and Perm Solutions. The recognized epitopes change depending on the method of fixation and permeabilization. I don't know which "homebrew" recipe may be compatible with all those clones. Jens
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