I'm studying honours in the Dept. Pathology and am looking at depolarisation of the mitochondrial membrane as an early event in apoptosis of Jurkat T cells. I'm staining cells with the mitochondrial specific dye Rh123 and am using 5microM staurosporine treated cells as a positve control to show mitochondrial depolarisation. However, staurosporine treatment appears to be causing an increased uptake of Rh123. I'm wondering if this is due to mitochondrial changes or another property of staurosporine treatment that is increasing uptake of the dye. If anyone has any experience with this area or any ideas, feedback would be muchly appreciated. Thanks, Anna Hearps (ahearps@postoffice.utas.edu.au) -- Mark A Cozens Division of Pathology University of Tasmania ph 03 62264828 fax 03 62264833
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