Hi Bernie! Pharmingen sells positive control cells called "Mick cells" (Mouse Intracellular Cytokine) which are mouse lymphoid cells which have been stimulated, fixed and non-permeabilized and express easily detectable levels of various cytokines. I believe they come in several flavors depending on the cytokine of interest. MICK-1 would work for gamma IF (cat. no. 20131Z) and MICK-2 (cat. no. 20132Z) for IL-4. Joanne Joanne Thomas, M.S. Director of Operations TRITECH Field Engineering 2014 Renard Court, Suite I Annapolis, MD 21401 1-800-886-7004 (USA) 1-410-266-1522 410-266-0935 (FAX) thomas@tritechinc.com -----Original Message----- From: bernie <bellette@postoffice.utas.edu.au> To: cyto-inbox Date: Wednesday, September 06, 2000 4:49 PM Subject: Intracellular Cytokine Staining > >Work in my department involves determining the intracellular cytokine >stores of murine CD4 and CD8 cells. Following staining and >assessment via flow, results to date have not demonstrated many >positive cells, even in cell lines known to be producers of the two >cytokines we are assaying for, IL-4 and IFN-gamma. Would anyone have >any trouble shooting tips or know of any cell lines that are >producers of these cytokines that come pre-stained for intracellular >cytokines that we can use as a positive control. > >Many thanks >-- >Bernadette Bellette >PhD Student >Discipline of Pathology >University of Tasmania > >(03) 6226 4762 (phone) >(03) 6226 4833 (fax) >
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