Question There is an effect that has been observed regarding the CV of 2.5 micron calibration beads using on a flow cytometer. When the peak channel is located at lower channel numbers the CV is increased. i.e. the CV of a population is higher at mean channel 100 ( 2.41) than at mean channel 800 ( 1.25). What is the statistical explanation for this phenomenon. Does it have to do with less channels being occupied when the mean is at lower numbers yeilding a higher CV? Bob Robert M. Zucker, PhD U.S. Environmental Protection Agency MD 72 National Health and Environmental Effects Research Laboratory Research Triangle Park, North Carolina, 27711 Tel: 919-541-1585; fax 919-541-4017 e-mail: zucker.robert@epa.gov
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