Dear Fellow Flowers, Recently our lab has begun analyzing cells from mouse lung mashes and BAL washes after virus challenge. It seems that we are seeing lymphocytes or cells in the "lymphocyte" gate display higher than normal levels and percentage % for certain T cell activation markers using monoclonal antibodies directly conjugated to PE(We typically pre-block with 4G2(Fc block) before mAb staining). My question is whether or not anyone has seen a similar effect with mouse lung cells, and whether this might be due to non-specific PE binding by contaminating cells in the "lymphocyte" gate. Respectfully yours, Jim Martin
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