Hello fellow flowers, I don't have access to a sorter or a MACS but I need to isolate B cells (ideally CD21+) from, rabbit, marmoset, and rhesus monkey whole blood. I've thought about coupling human C3d (the ligand to CD21) to sepharose then passing either buffy coat or lysed blood prep, washing the unbound CD21- cells and eluting the CD21+ cells... Any other suggestions would be appreciated. If MACS or sorting is the only other possible way then I could probably get access to these pieces of kit....any suggested protocols would be greatly appreciated.. Thanks, Louise Dr. Louise Affleck Senior Scientist Protein Function Group AdProTech Ltd Unit 3, 2 Orchard Road Royston Herts SG8 5HD Tel: +44 (01763) 226090 Fax: +44 (01763) 226021 http://www.adprotech.co.uk
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