Dear Dr. Gaudry, hi Flowers, islands of Langerhans (from rats) were sorted with a purity of 90% on a Partec PAS flow cytometer (http://www.partec.de) equipped with the large particle version of the closed piezo sorter. [D.W.R.Gray et al.: Separation of Pancreatic Islets by Fluorescence-Activated Sorting, DIABETES, Vol. 36, Suppl. 1, (1989)]. The islands were stained by neutral red, analyzed and sorted by fluorescence only, and stayed viable and functioning after sorting and tranplantation beneath the kidney capsule of rats. With best regards Wolfgang Göhde jun., Partec GmbH *********************************** partec GmbH Otto-Hahn-Str. 32 D-48161 Münster, Germany phone: ++49 - 2534 - 8008 0 fax: ++49 - 2534 - 8008 90 e-mail: info@partec.de WWW: http://www.partec.de *********************************** -----Ursprüngliche Nachricht----- Von: L.gaudry@unsw.EDU.AU [mailto:L.gaudry@unsw.EDU.AU] Gesendet: Montag, 28. August 2000 02:04 An: Cytometry Mailing List Betreff: Islets of Langerhans Hi, Would anyone have a procedure for flow sorting of the islets of Langerhans? I have been told that they are difficult to detect on forward and side scatter because there is a lot of other tissue bits about the same size. Is there some antibody that can be used to label the islets? Thank you, Leonie
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:30 EST