Hi all, We have been having some problems with some of our monoclonals and would appreciate any advice or comments... Bascially we have two problems which may be related: after purification on Protein A or G and elution (glycine pH2.5 then tris pH8 neutralisation and dialysis against PBS), the antibody either precipitates spontaneously at 4 degrees, or the biotinylated conjugates rapidly lose activity (which may also be due to precipitation - we haven't checked yet). We suspect low levels of acid denaturation may cause ongoing spontaneous formation of aggregates. Does anyone have any experience of this and how to get around it (most mAbs are fine after purification this way and only some mAbs seem to be affected)? Thanks, Adrian -- ****************************************************** Adrian Smith (PhD Student) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. ******************************************************
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:30 EST