BrdU Hoe Quenching

From: Lindemann, Carsten (CLindemann@eufets.com)
Date: Wed Aug 30 2000 - 06:56:07 EST

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I have two questions about the protocol for the BrdU/Hoechst Quenching
assay.
Is it sufficient to permeabilize cells by using NP40 (0,5%) buffer in order
to stain nuclear DNA?
Is fixation with ethanol recommended or does Eth disturb Quenching of
Hoechst dye?

Thanks for any comments

Carsten Lindemann
EUFETS GmbH
Germany

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