Dennis Young was kind enough to let me know that my attachment didn't get attached, so here is my question again with the histogram. Thanks in advance for any comments. I am gating through area vs. width on a FACSCalibur. Candace ____________ >Dear Flowers, > >I have attached a simple text picture of a cell cycle analysis on COS7 cell >line that was serum starved for 3 days. COS7 is apparently a double >nucleated kidney cell line. This is 24 hours after being serum stimulated, >but all the historgrams give this S-phase peak, or at least I have been >analyzing it as S-phase, since the third peak is at the appropriate G2M >position. Has anyone had experience with COS7 or other double nucleated >cell cycles that can enlighten me? > >Thanks, >Candace Enockson >Medical University of South Carolina > >---------- End Forwarded Message ---------- > > > > ---Dennis Dennis J. Young Flow Cytometry Core Facility University of California, San Diego Internal Medicine Group, Bldg #4, Rm 126 9500 Gilman Drive La Jolla CA 92093-0671 Mail:<<mailto:djyoung@ucsd.edu>> WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>> Telephone:(858) 822-0407 FAX: (858) 822-0403 ---------- End Forwarded Message ----------
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