smooth muscle cells

• Next message: Joanne Thomas: "Fw: APC 0344 response"
• Previous message: Jian G Zhang: "Rat IL-4"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

I am trying to use FACs analysis on rat aortic smooth muscle cells.
Unfortunately the cells do not detach with EDTA/no Mg,Ca conditions, and if I
force them off with a high velocity stream of media, many of the cells are
damaged.  I am trying to detect a cell surface proteoglycan, so I do not want
to use trypsin to detach the cells.  Does anyone have any ideas as to what I
can use to get the cells in suspension?  Does anyone have any personal
experience with rat aortic smooth muscle cells and FACs?
If you have any suggestions, please email me at michelle.houston@usa.net.

Thank you.

Michelle Houston

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1

• Next message: Joanne Thomas: "Fw: APC 0344 response"
• Previous message: Jian G Zhang: "Rat IL-4"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:29 EST