100,000g for a mininum of 45 mins will get you monomeric IgG, in fact will leave you with aqueous phase proteins. Use the top 2/3 of the tube to be on the safe side. If you are worried about large aggregates (such as in sera) then a microfuge set at 13000 rpm (high speed) for 5 min is enough. Craig Turner Does anyone ultracentrifuge their labeled primary antibodies or MHC tetramers before use in cell surface staining? If so, can someone pass along what conditions you use (ie how many g's and for how long)? We have a desktop airfuge. I can't find a chart anywhere telling what size proteins pellet at what speed. If anyone has this info, I would greatly appreciate it. Thanks Diana Martin Post-doctoral Fellow Center for Tropical and Emerging Global Diseases University of Georgia ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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