At 11:22 11/08/2000 +0100, you wrote: > >Dear Fellow Flowers, especially UK ones, >how do you dispose of your waste when contaminated with propidium iodide? Is >there anybody that can recommend protocols or treatments to allow the >filtered buffer to be safely disposed of without environmental risk? >Thanks again for your help as usual, and I'll post a summary of the replays. > >Cheers, Mara > Several standard method for removal of ethidium bromide toxicity have been described in the classic 'Molecular Cloning' by Sambrook et al.. (pp. E.8-E.9). Since PI and EB have very similar structures I suppose these methods apply to both. The possibilities are: A. for concentrated solutions: 1. use of nitrite in acid solution 2. to use permanganate in acid solution (both methods are based on oxidation of the planar aromatic rings responsible for DNA intercalation) B. for diluted solutions: Use absorbent (e.g. activated charcoal) to remove the EtBr from the solution Cheers, Joeri ------------------------------------- Joeri Aerts Experimentele Laboratoriumgeneeskunde Laboratoriumgeneeskunde (Hematologie) CDG 7e VD - Gasthuisberg Herestraat 49 B-3000 Leuven BELGIUM tel.: +32-16347012 fax.: +32-16347042 e-mail: joeri.aerts@uz.kuleuven.ac.be -------------------------------------
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