Hi I've stained a lot of cells from people on various HAART therapies and not found any unusual autofluorescence. Anthracyclines used in tumor therapy are brightly fluorescent. Are you seeing real autofluorescence in cells without any staining or is it non-specific binding of your antibodies? I've seen non specific binding of all antibodies after monkeys and humans have been treated with anti CD3 and CD8 antibodies due to an humoral immune response to the "theraputic " antibodies. Washing cells before staining prevented it. Good luck Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> "Bryant, Jenny (NSW)" <JBryant@arcbs.redcross.org.au> - 8/11/2000 2:34 AM >>> Hello to all, I have just tested a patient on antiretroviral therapy whose cells fluoresced to the extent that compensation was impossible. This has prompted 2 questions, 1. Does anyone know of a list of drugs (prescribed or "recreational") which cause this phenomenon? 2. How do you deal with these patients? Do you lyse and wash before staining? If so, how would this effect your results? Thanks Jenny Jenny Bryant Flow Cytometry Australian Red Cross Blood Service - NSW/ACT 153 Clarence St Sydney, NSW 2000, AUSTRALIA .._|\ Ph: 61 2 9229 4341 / \ FAX: 61 2 9229 4521 \_.-._/<<<<<< E-mail: jbryant@arcbs.redcross.org.au v
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