Hi everyone out in Flowland, I have a problem I hope someone can help me with. I have an investigator who is looking at apoptosis in cultured human myoblasts with myotonic dystrophy using Annexin-V/PI. The problem I am having is that in the Annexin V-FITC only sample, I get a signal in the PI range. I've tried to compensate, but even at 100% I still have some signal. Looking at the cells under a scope show some faint red staining within the cell. The first time I ran there cells I had this problem and I thought that it might have been cross contamination (the kit they are using is a shared one), however, they bought a new kit and the problem persists. The investigators add human epidermal growth factor to their media. Could this be a cause of the PI signal? Any help would greatly be appreciated! Thanks! Andy (:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:) Andy Oberyszyn, M.S. The Ohio State University Analytical Cytometry Laboratory 416 Comprehensive Cancer Center 410 West 12th Avenue Columbus, Ohio 43210 Tel: 614/292-FLOW(3569) Fax: 614/292-7335 E-Mail: cytometry@osu.edu Web Page: <http://cytometry.med.ohio-state.edu>http://cytometry.med.ohio-state.edu (-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-) "What if the Hokey Pokey is really what it's all about?!?"
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