Response to Thanos Kakoulidis EDTA works on most adherent endothelial cells, but takes time. TIming is critical because the cells are not altogether happy. We have on occasion (e.g. for porcine embryonic endothelial cells) used EDTA + collagenase. Bob Auerbach At 07:09 PM 8/7/00 -0400, you wrote: >Hi Flowers, > >does anybody know of another method except trypsinization, to detach >endothelial cells from the flask? (that is necessary in order to create a >single cell suspension and run the cells in the flow cytometer) > >thanks, > >thanos > >Thanos Kakoulidis, MD >Ph.D. Graduate student >Department of Microbiology and Immunology >Institute for Cellular Therapeutics >University of Louisville Robert Auerbach Laboratory of Developmental Biology University of Wisconsin 1117 West Johnson Street Madison, WI 53706 Phone: 608 262 1789 FAX 608 262 1171 e-mail rauerbac@facstaff.wisc.edu (formerly auerbach@macc.wisc.edu)
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