Hi We have chosen biotin-streptavidin system to detect low expression membrane surface molecules. We acquired biotinylated antibody and Sv-PE from Pharmingen (BD), but in the product bulletin there aren't the start concentration of Sv-PE to be used in the test. If anybody has some experience, please help us Thanks in advance Margareth Universidade Federal de São Paulo/EPM Disciplina de Hematologia e Hemoterapia E-mail: margh@hemato.epm.br
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