Re: CD4/CD8

From: Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Date: Wed Aug 09 2000 - 12:37:01 EST

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It is normal to find a population of CD4-CD8- T cells. Gamma delta T
cells are either CD8+ or double negative.

	Maryalice
>We are using a 4-color mix of CD45/CD3/CD4/CD8 to determine T cell subset
>ratios. However our CD4 + CD8 percentages (gated on the CD45+ CD3+ cells)
>do not add up to 100%. Has anyone else had these problems or have any
>suggestions? Help would be appreciated - thanks!
>Lisa Whyte

Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH

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