Jackson Immunoresearch has an abundance of secondary Abs that are F(ab')2 fragments that don't bind to Fc receptors AND have also been adsorbed against multiple species. I have had excellent results whenever I have used their antibodies. It sound like you have a real problem with your current secondary Ab and I strongly suggest you try one of Jacksons. The other company that has some isotype specific secondary Abs is Southern Biotechnology Associates, Inc. , phone: (205) 945-1774. We just bought a goat anti-mouse IgG1-HRP conjugate for Westerns and it was great. You can try any fluorescent combo you want. Your problem is your background staining with the FITC-conjugated secondary. Secondary only should never be so high. Try out the above companies. I am certain it will improve your results. Good luck. Dr. Susan J. Zunino Lehrstuhl fur Genetik University of Erlangen Staudtstr. 5 91058 Erlangen Germany Tele. 49 9131 8528784 FAX 49 9131 8528526 e-mail: szunino@biologie.uni-erlangen.de
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