Dear Flowers Hello and regards to all Thanks to the members of this group I got some good answers to my queries. Thanks a lot. I was going through a protocol for viability assay with trypan blue (from the Purdue University's CD-ROM for flow cytometry. I wanted to know the emission spectrum of Trypan blue and what is the composition of the dye. Sigma sells 2 of them one a powder T0776 aprox 70%dye content and the other a liquid 0.4% T8145. Which of these is most suitable for the assay.The fluorescence is checked on FL3. I would be grateful for any help regarding this. Thanks. Rana Nagarkatti ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
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