In cell cultures, cells not infrequently endoreduplicate. There will then be a tetraploid cell cycle imposed on the diploid cell cycle. You can most clearly demonstrate endoredupliation by staining for cyclin B1. I have assumed that you have eliminated cell clumps from the analysis, these are the most trivial explanation for a DNA content >G2. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: +44 (0)1737 241726 FAX: +44 (0)1737 226736 Mobile telephone: 07802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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