Large cells and DNA analysis

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I have a client that wants to run very large cells(about 300 um in size) in
our FACSCalibur and wants to do DNA analysis on these cells, some of which
have 30-40 nuclei per cell.   I don't have a great deal of experience doing
DNA analysis.  Is it possible to 1) run cells this large through the flow
cytometer and
2) quantitate 30-40 nuclei per cell???

I only have experience looking at 3-4 nuclei per cell

I would throughly appreciate any help that anyone may offer.

thanks in advance for your advice.

Sincerely

Janet Dow

Janet Dow
Research Technician and Manager
Flow Cytometry Facility
North Carolina State College of Veterinary Medicine
Room C-314
Raleigh, NC 27606
(919)513-6364

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