Mosiman, V.L. et. al. Reducing Cellular Autofluorescence in Flow Cytometry: An In Situ Method, Cytometry 30:151-156, 1997. kb -- Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida Phone: (352) 392-4887 Fax: (352) 392-5393 kbahjat@ufl.edu > From: "Geneva M. Omann" <gmomann@umich.edu> > Date: Fri, 28 Jul 2000 09:40:43 -0500 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Fw: trypan blue to reduce background > > > Hello Flowers, > Along the lines of Shannon Ritchie's question below, I have also heard > of using trypan blue or crystal violet to quench the fluorescence of > externally bound ligands. I believe the mechanism is by energy transfer. > Has anyone else heard of this and if so do you have references for the > mechanism and protocols? > > ----- Original Message ----- > From: Shannon C. Ritchie <sritchi@emory.edu> > To: cyto-inbox > Sent: Wednesday, July 26, 2000 1:42 PM > Subject: trypan blue to reduce background > > >> >> I have heard of using trypan blue to reduce background. Does anyone know >> the concentration and protocol? >> >
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