Len, others . . . Whether to wash or not following annexin V binding is probably more important for adherent cell types -- I do know this is true for us. MAK. > On Sun, 23 Jul 2000, Len Brown wrote: > > What is the consensus as to "wash" or "not-to-wash" human blood cells after > > Annexin-V FITC staining. Most protocols do not wash. If you wash in what > > buffer, Annexin Ca++ binding buffer, PBS etc.? This subject has been touched > > on briefly in the past but not resolved. > -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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