>Date: Wed, 19 Jul 2000 09:19:33 +0530 >From: Ranga Uday Kumar <uday@jncasr.ac.in> >Organization: JNCASR >X-Accept-Language: en >MIME-Version: 1.0 >Subject: CD 16 depletion from PBMC >X-PMFLAGS: 34078848 0 1 2221.CNM >Status: > Please send your answers to : uday@jncasr.ac.in > >Hi netters, > > This question is slightly off the track. Hope some one will respond. >I would like to remove CD16 positive cells from freshly isolated human >peripheral blood leukocytes by panning the cells on plastic surface with >specific antibody immobilized. The idea is to deplete PBL of NK cells. >(I don't have access to FACS facility). Several hybridomas are available >from ATCC. Has any one used any of these hybridomas for this purpose? >Your suggestions and recommendations are appreciated. > >-- >________________________________________________________ >Ranga Uday Kumar Ph.D. >Faculty Fellow >Molecular Biology and Genetics Unit >Jawaharlal Nehru Center for Advanced Scientific Research >Jakkur Post, Bangalore 560 064 >India >Tel: +91 (80) 846 2750 Ext. 241 >Fax: +91 (80) 846 2766 >________________________________________________________ > ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:26 EST