Hi Everyone, I have a researcher who has an interesting question that I do not have an answer for. I was hoping someone could help since at this moment I just do not have the time to go do the research. He is not on the Cytometry Mailing List so please email him directly at Bernardo Trigatti <bltrigat@MIT.EDU> . Thanks, Glenn MIT My question is whether I can make use of the possibility of transfering male bone marrow into lethally irradiated female mice (bone marroy ablated) to detect donor (male) bone marrow derived cells in the female recipients. The reason that I would like to do this is to have a way of monitoring the timecourse of repopulation of bone marrow-specifically bone marrow derived macrophages in the recipient females. What I want to know is whether there is any way of detecting cells containing a Y-chromasome, or any other way of differentiating male from female cells. Dino Bernardo Trigatti, Ph.D. 68-471 Krieger Lab Biology Department Massachusetts Institute of Technology 77 Massachusetts Ave Cambridge, MA 02139 USA Tel: (617) 253-8802 Fax: (617) 258-5851
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