Phoenix flow systems makes a reagent for disassociating adherent cell types. You can find some information at http://www.phnxflow.com/. I believe they will even send a free sample if you pay the shipping. -Dave ************************************************************* David Novo Dept. of Physiology CHS C8-134 (310) 206-8160 ************************************************************* On Tue, 18 Jul 2000, Janet Konyer wrote: > > > Hello, > > Would anyone be kind enough to suggest some techniques for analyzing > adherent cell types by flow? Specifically, we are looking at a human cell > line of promyelocytes, which when treated with phorbol esters > differentiate into monocytes & macrophages. We are attempting to analyze > changes in CD 14 expression. We are having problems with the cells > adhering to one another after trypsin treatement to lift the cells from > the plates. > > many thanks, > Janet > >
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