fixing YFP+ cells

From: Lisa Hunt (hunt@dcsun1.epfl.ch)
Date: Tue Jul 18 2000 - 12:14:44 EST


I would like to analyze the per cell fluorescence of CHO cells
expressing YFP over an entire culture.  Any suggestions - for a fixation
that won't totally kill the fluorescence and will maintain it stable
for, say, 2 weeks so that I can analyse all the samples at the same
time, under the same conditions?  Or any alternative (better/easier)
suggestions?
Thank you in advance!
Lisa



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