Re: T DISORDER

From: Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Date: Wed Jul 12 2000 - 09:47:16 EST


This is a T cell neoplasm. The convolute nuclei, CD4+ and CD7- makes
one think of ATL associated with HTLV1 or mycosis fungoides- Sezary
syndrome. Are the nuclei cerebriform or flower shaped? What about
CD25? The morphology nd clinical data help to distinguish. Great case!

	Maryalice

>I have a sample from a woman, 59 years, walking, with the diagnosis of
>lymphoma.
>Dont have adenopaties, lttle splenomegaly, with gladder stone, total
>bilirrubin 4.5 mg/dl, GOT, GPT a and Alcaline fosfatase high.
>The haematolgical parameters are : WBC "113.000", Plt 53.000, Ht 39.6%,
>Hb 13.7% . 80% of elements of size regular, convolute nuclei, with one
>ocassionaly nucleoli. Agranular and basophil citopllasm.
>The CF show a population of 87 % of cells that have a SSC, FSC and CD45
>normal. Also are CD19(-), CD20(-), CD34(-), CD33(-), CD13 (-), CD14 (-),
>CD10 (-), Tdt (-), MPO (-), and CD3(+++), CD4 (+++), CD5 (+++), CD2(-),
>CD7(-), CD8(-), HLA-DR (++) (58 %), NK(-).
>This population, CD3(+++), have a little porcentaje that are very brigth
>(1.51%) and are CD2(+++), CD7(+++), CD5(+++), CD4(++)(52.5%),
>CD8(++)(44.09), ratio CD4/CD8=1.1, and HLA-DR(+)(29.3%). The big
>population that are CD3(+++)dim (really more than dim) are CD4(+++),
>CD8(-), CD2(-), CD7 (-), CD5(+++) and HLA-DR (++)(57.6%).
>
>Opinions?
>
>Thanks
>--
>JUAN LUIS CASTILLO N.
>CITOMETRIA DE FLUJO
>HOSPITAL DEL TRABAJADOR
>CARDENIO AVELLO 36
>CONCEPCION
>CHILE
>PHONE:  56-41-402138
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>http://oncoinmun.bizland.com/

Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH



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