Dear Flowers, I have a client who is trying to detect Topoisoerase IIa, currently in HL60 cells, ultimately in leukemia cells. So far, we have been unable to detect them and feel certain it is a permeabilization problem since Topo is expressed intra- nuclearly. Has anyone got a protocol for such staining? Thanks in advance, Candace Enockson Medical Unversity of South Carolina
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