Hi Colin We isolated mouse Kupffer cells by collagenase perfusion-digestion in this publication: Lee SH, Crocker PR, Gordon S. Macrophage plasma membrane and secretory properties in murine malaria. J Exp Med. 163:54-74. 1986. Best regards Szu-Hee Lee Dept of Pathology National University of Singapore -----Original Message----- From: Tom Mc Closkey [mailto:thomasm@nshs.edu] Sent: Thursday, June 29, 2000 3:31 AM To: cyto-inbox Subject: Re: Isolation of kupffer cells arturo Colin Martinez wrote: > > I´m looking for information on how isolate kupffer cells from mouse´s liver > and spleen. If any one can direct me to appropriate references and/or has > any practical suggestions, I would greatly appreciate hearing from you. You might find the following reference useful: Mc Closkey, TW, et al. Lipopolysaccharide treatment of rats alters antigen expression and oxidative metabolism in hepatic macrophages and endothelial cells, Hepatology, 16: 191-203, 1992. This work was done in a rat model system but describes Kupffer cell isolation and the method should be similar. Good luck, Tom **************************************************************************** * Thomas W. Mc Closkey, Ph. D. Director of Flow Cytometry, North Shore University Hospital Assistant Professor of Pediatrics, New York University School of Medicine Boas Marks Biomedical Research Center, 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866 **************************************************************************** *
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