Dear list, I am interested in looking at the expression of fibroblast growth factor receptors (transmembrane) in bone cells. I have primary antibodies to the 4 FGFRs that recognise the C terminal tail (cytosolic). Therefore, I need to permeablise the cell membrane to allow the antibody to bind. Does anyone have a protocol suitable for FGFRs? Also, most protocols call for 1 million cells to be divided into 50 microlitre aliquots for fixing and staining. Is this a sufficient number of cells per 50 microlitre aliquot or should I use more? Thank you in advance. simon ---------------------------------------------------------------------------- Dr Simon Cool Department of Anatomical Sciences University of Queensland Brisbane, Q4072 Australia PH:+61 7 3365 4593 FAX:+61 7 3365 1299 Email: s.cool@mailbox.uq.edu.au ******************************************************************** This e-mail is confidential and may be privileged. It may be read, copied and used only by the intended recipient. If you have received it in error, please contact the sender immediately by return e-mail or by telephoning +61 (7)3365 4593. Please then delete the e-mail and do not disclose its contents to any person.
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