>Hi, > >I injected some EBVLCL's into mice and stained for human and mouse CD45 and >it looks like there is some cross reactivity. I was wondering if there is a >human CD45 antibody clone that does not cross react with the mouse CD45? > >Thanks, > >A Chaudhuri PhD >Hematology Branch, >NIH I have routinely use Pharmingen's antibodies to mouse and human CD45 (FITC and PE conjugates) together to stain cells recovered from hu-SCID mice, without seeing any cross-reactivity. You may be seeing non-specific binding to the LCL due to high expression of FcR, which should be overcome by blocking with Ig of the appropriate species. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Mandy Cromwell, Ph.D. Research Fellow Department of Immunology New England Regional Primate Research Center Harvard Medical School One Pine Hill Drive Southborough, MA 01772 (508)624-8022
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