Hi, For those who have experience cloning cells, what do you do to insure that single cells, cloned by FACS into 96-well plates, continue to grow? Add factors (if so, which) or cell growth-conditioned medium? Although any suggestions in general will be appreciated, the cells in question at the moment are 293 cells (human kidney carcinoma, I believe). Any advice would be appreciated. Thanks. Ray Raymond B. Hester, Ph.D. Research Cytometry Laboratory CSAB 357, Biotechnical Services University of South Alabama Mobile, AL 36688 Voice: (334) 460-6029 FAX: (334) 460-6073 E-mail: rhester@jaguar1.usouthal.edu
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