Yes. Don't do it. We tried and found the technique too undependable. We found we can't use frozen cells and the slightest variation in conditions results in either false positives or false negatives. Therefore you can never have confidence in the results. We found DNA content works better- mantle cell almost always has cycling cells while CLL (the other big time CD5+ B cell neoplasm) never does. Maryalice >Does anyone have experience in evaluation of expression of cyclin D1 >in mantle cell lymphomas by flow? > >Thanks in advance > >Martini Giuseppe > > > > > >Do you know MailStudio? Powered by <http://www.3rsoft.com>3R Soft, Inc. > > > Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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