Hello all... A colleague sent me this query. Any insight/advise? Thanks! Bill Telford _____________________________ >Dear Bill, > >A colleague wanted to perform flow cytometry studies of the uptake of >molecules in RBC's. It would appear to me that it would be problematic >with fluorescein/rhodamine due to the strong hemoglobin absorption in the >RBC. Do you have any idea about how long wavelength fluorophore [cy5 or >Texas Red] is required for quantitative measures of RBC uptake (not ghosts >but normal rbc's)? Do you know of anyone who has done RBC uptake >measuremetns with fluorescent labels by flow cytometry? > >Thanks, >Bob
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