DNA ON PARAFFIN-EMBEDDED TISSUE--PLEASE POST

From: Saldivar, Victor (victor_saldivar@srhc.iwhs.org)
Date: Thu Jun 15 2000 - 17:10:31 EST


Colleagues in FLOWCYTOMETRY: Every now and then we go back to having
problems with obtaining good CVs (and reliable results) on deparaffinized
tissue for DNA.
My FLOW Supervisor requested to post th following question: "?Any  (new)
ideas about the steps to get the paraffin embedded tissue to a single cell
suspension?".
We will appreciate any "pearls".
Victor A. Saldivar, MD -- San Antonio, TX
<victor_saldivar@srhc.iwhs.org>



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