Re: interferon gamma Abs/TNF-a ELISA for NHP

From: mandy cromwell (mandy_cromwell@hms.harvard.edu)
Date: Tue Jun 13 2000 - 14:04:19 EST


Biosource International (www.biosource.com) offers ELISA kit for
rhesus IFN-g and TNF-a as well as other cytokines. Most if not all of
these will work for cynos as well-I know from experience that IFN-g,
TNF-a, IL-2 and IL-12 x-r with cyno. It may be possible to buy the
IFN-g Abs separately for other applications-you didn't specify what
you wanted to do with the Ab. Pharmingen's anti-IFN-g works well for
ICC in rhesus cells, but I don't know if it works for cyno.
good luck!

Mandy Cromwell, PhD
Immunology Division
New England Regional Primate Research Center
Southborough, MA
(508)624-8022

>Perhaps I am looking in all the wrong places, but I cannot find any
>anti-interferon gamma antibodies that cross react with cynomolgus monkey.
>Anyone know of any or have any good protocols? Much obliged to all.
>
>Amelia Bartholomew, MD
>Assistant Professor, Surgery
>Division of Transplantation
>840 South Wood Street
>Chicago, Illinois  60612
>phone: 312-996-6771
>fax: 312-413-3483



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