Re: Sunnyvale Red

From: Robert Johnson (johnson@caltag.com)
Date: Thu Jun 08 2000 - 09:27:42 EST


Dear Arnold

Red670 is, of course, a PE-Cy5 tandem like our Tri-Color or Pharmingen's
CyChrome.  If Sunnyvale Red is a single dye exciting at 488 and emitting at
670, it would be like B-D's PerCP.  It would be great if a good alternative
existed.  We would certainly be interested
Regards
Bob Johnson
Caltag

Arnold Pizzey wrote:

> At 10:53 02/06/00 -0700, you wrote:
> >
> >Hi all,
> >
> >Our HTS department recently gave a presentation where they described as
> >assay system using a fluorochrome named Sunnyvale Red.  It excites at 488
> >nm and has a emission wavelength of 670 nm.  Is anyone familiar with it
> >and can it be used for flow?
> >
> >Thanks,
> >
> >Dixie Polakoff
> >FACS operator
> >Protein Design Labs
> >(510) 574-1528
> >
> >
> >
>
> Greetings Dixie,
>
> Sounds to me that this might be 'RED670'; I have used Streptavidin-RED670
> for the enumeration of G-CSF receptors via biotinylated G-CSF. I estimate
> that this fluorochrome is capable of reporting <100 receptors.
> It is available from GibcoBRL cat no.19543-016
>
> regards
>
> Arnold
>
> _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
>         Arnold Richard Pizzey
>         Department of Haematology
>         Royal Free and University College London Medical School
>         98 Chenies Mews
>         London WC1E 6HX
>         U.K
>
>         voice:  +44 020-7679-6234
>         Fax:    +44 020-7679-6222
>         email:  a.pizzey@ucl.ac.uk
> _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/



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