GAPDH is my choice for a good housekeeping gene. Yes, I always try to use primers that span an intron (or a few) if I can. Also, you could run a PCR reaction with RNA only to see what level of genomic DNA contamination you might have. Dr. Susan J. Zunino Lehrstuhl fur Genetik University of Erlangen Staudtstr. 5 91058 Erlangen Germany Tele. 49 9131 8528784 FAX 49 9131 8528526 e-mail: szunino@biologie.uni-erlangen.de
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