Hi everyone I want to quantitate CD38 expression on T lymphocytes. Giorgi et al have described a method to do this using the CD4 expression as a biological calibrator. In their system they estimated the number of PE molecules per relative fluorescence intensity unit (RFI) to be 41, assuming the number of anti-CD4 molecules per cell to be 50.000. If I want to use this method I assume I have to use an anti-CD4 and anti-CD38 with a 1:1 conjugate ratio and also to calculate the RFI. My questions are: 1.- How can I convert the cyometer report values (Mn of X and Mn of Y) to RFI?; 2.- Where can I get the 1:1 conjugates? Thanks Jose Miguel Benito Hospital Carlos III Madrid Spain ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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