Greetings: We are starting to sort human CD34+ cells for subsequent transduction, so viability is a premium. We are interested in learning what others have found in regard to maintaining viability. The cells have been stored in LN2, 10% DMSO. We have been analyzing the cells with the Coulter Stem-Kit and have built a sort protocol from the assay. What types of media? Are cells sorted directly into an induction medium? Any effect with pressure or flow rate? If you can share a publication or reference source, we would appreciate the literature citation. Thanks to everyone for the wonderful discussions. Barbara Breithaupt Research Associate, HGTRI Clinical Applications and Stem Cell Laboratory 515 241-5162 515 241-8788 FAX
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