Hi, I do surface labeling with subsequent fixing and internal labeling all the time and it works for me. I permeabilise with lysolecithin and 3.7% paraformaldehyde. Don't know whether it would work for all surface antigens. Deb Berglund Montana State University On Mon, 29 May 2000, Bratislav Janjic wrote: > > Dear fellows, > > Is there any possibility to perform simultaneous labeling (I mean, in the same sample) > of surface and intracellular antigens? Is it possible, for example, to perform labeling > of monocytes with CD14, and after that, to permebilise them and label intracellular > antigens (for example caspases)? Does the cell permeabilising affect labeling of > surface antigen? If somebody has experience with similar procedures, please contact me. > > Thanks in advance. > > Bratislav Janjic, BS > Department of Pathology > University of Pittsburgh > > > > ___________________________________________________________________________ > Visit http://www.visto.com/info, your free web-based communications center. > Visto.com. Life on the Dot. > >
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